Non-descanned multifocal multiphoton microscopy with a multianode photomultiplier tube
نویسندگان
چکیده
منابع مشابه
Non-descanned multifocal multiphoton microscopy with a multianode photomultiplier tube.
Multifocal multiphoton microscopy (MMM) improves imaging speed over a point scanning approach by parallelizing the excitation process. Early versions of MMM relied on imaging detectors to record emission signals from multiple foci simultaneously. For many turbid biological specimens, the scattering of emission photons results in blurred images and degrades the signal-to-noise ratio (SNR). We ha...
متن کاملMultifocal multiphoton microscopy based on multianode photomultiplier tubes.
Multifocal multiphoton microscopy (MMM) enhances imaging speed by parallelization. It is not well understood why the imaging depth of MMM is significantly shorter than conventional single-focus multiphoton microscopy (SMM). In this report, we show that the need for spatially resolved detectors in MMM results in a system that is more sensitive to the scattering of emission photons with reduced i...
متن کاملMultifocal multiphoton microscopy.
We present a real-time, direct-view multiphoton excitation fluorescence microscope that provides three-dimensional imaging at high resolution. Using a rotating microlens disk, we split the near-infrared light of a mode-locked titanium-sapphire laser into an array of beams that are transformed into an array of high-aperture foci at the object. We typically scan at 225 frames per second and image...
متن کاملStochastic scanning multiphoton multifocal microscopy.
Multiparticle tracking with scanning confocal and multiphoton fluorescence imaging is increasingly important for elucidating biological function, as in the transport of intracellular cargo-carrying vesicles. We demonstrate a simple rapid-sampling stochastic scanning multifocal multiphoton microscopy (SS-MMM) fluorescence imaging technique that enables multiparticle tracking without specialized ...
متن کاملCharacterization of a multianode photomultiplier tube for use with scintillating fibers to detect gamma rays
The performance of a multianode photomultiplier tube (MAPMT) with 64 anodes coupled to scintillating fibers has been examined. Such a detector system can be used in a pairproduction gamma-ray telescope as well as in other applications. The characteristics of these tubes (Hamamatsu R5900-00-M64) are presented including single photoelectron sensitivity, electrical and optical cross-talk, and dark...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: AIP Advances
سال: 2015
ISSN: 2158-3226
DOI: 10.1063/1.4916040